Broncial Washings Cytology

NAME OF PROCEDURE

Broncial Washings Cytology

TEST CODE

NGYN

CPT CODE

  • 88112 – Cytopathology, selective cellular enhancement technique with interpretation (eg, liquid based slide preparation method), except cervical or vaginal
  • 88305 – Level IV Surgical pathology, gross and microscopic examination.

SYNONYMS

Washing Cytology

APPLIES TO

Bronchial-Alveolar Lavage Cytology; Bronchial Lavage/Wash Cytology; Tracheal Lavage/Wash/Aspirations Cytology.

SPECIAL INSTRUCTIONS

Information regarding type of specimen, admitting diagnosis, and pertinent clinical history (i.e. age, clinical impression, past diagnosis, radiographic findings, and history of radiation or chemotherapy) is essential to interpretation and should be noted on the requisition.  Clinical evidence of immunosuppression should be indicated.  Special handling requirements (isolation) should be clearly noted on the requisition.

SPECIMEN

  • Bronchial Washings
  • Volume: 5mL
  • Minimum volume 1-2mL

CONTAINER

Bronchoscopy collection apparatus (suction tube).  Please label submitted container(s) with a minimum of two patient identifiers.

COLLECTION TECHNIQUE

To collect bronchial washings, instill saline into the lung while the bronchscope is in place and aspirate the material.

Label the container with the patient’s name, date, and time of collection.

Transport the specimen to the laboratory as soon as possible.  Refrigerate the specimen if it cannot be processed promptly.

REQUEST FORM

Complete a Anatomical Pathology Requisition as indicated in the Test Ordering section of this manual. The patient information section as well as the Non-Gyn Cytology portion of the form should be completed.

CAUSES FOR REJECTION

Specimen container not properly labeled.

Prolonged period (more than 24 hours) at room temperature.

LIMITATIONS

Negative bronchial washing is considered nondiagnostic if respiratory epithelium is not present.

Negative findings for infectious agents may be nondiagnostic.

ADDITIONAL INFORMATION

Special stains may be necessary (i.e. silver procedures for Pneumocystis, mucicarmine for mucins) and performed when appropriate.

Post-bronchoscopy sputum for cytology should be collected as this specimen sometimes yields more diagnostic cells than are obtained during the bronchoscopy.

METHODOLOGY

Preparations are examined cytologically using the Pap stain method.