Brushings Cytology

NAME OF PROCEDURE

Brushings Cytology

TEST CODE

NGYN

APPLIES TO

Bronchial Brushings cytology, Colonic Brushings cytology, Esophageal Brushings cytology, Gastric Brushings cytology, Oropharyngeal Brushings cytology, Ureteral Brushings cytology, Urethral Brushings cytology

SPECIAL INSTRUCTIONS

Information regarding the site brushed, admitting diagnosis, and pertinent clinical data (i.e. age, clinical impressions, past diagnosis, radiographic findings, bronchoscopic findings, and history of radiation or chemotherapy) is essential to interpretation and should be noted on the requisition. Clinical evidence of immunosupression should be indicated where appropriate. Special handling requirements (isolation) should be clearly noted on the requisition. A separate request should be submitted for each site sampled.

SPECIMEN

Brush from lesion area (spray-fixed smears or brushes in fixative).

CONTAINER

Capable plastic container with Cytolyt cytology fixative (or normal saline if Cytolyt is not available) for submitting brushes, and/or cardboard slide holders and spray fixative.  Please label submitted container(s) with a minimum of two patient identifiers.

COLLECTION TECHNIQUE

  1. Collect specimen using brush
  2. If submitting slides, roll brush gently over glass slide to cover the area of a quarter.
  3. Fix immediately with spray fixative. NOTE: Optimum preservation is obtained if smears are prepared quickly without undue time spent spreading material. Smears should be sprayed within 1-2 seconds after spreading. The fixative should be held about 12 inches from the slide. A brief application of fixative is adequate.
  4. Place the brush in container of Cytolyt cytology fixative (or clean container containing sterile saline –several milliliters – to cover).

REQUEST FORM

Complete a Anatomical Pathology Requisition as indicated in the Test Ordering section of this manual. The patient information section as well as the Non-Gyn Cytology portion of the form should be completed.

CAUSES FOR REJECTION

Specimen container or slides not properly labeled.

LIMITATIONS

Allowing smears and brushes to dry before they are fixed will render them unsatisfactory for cytologic evaluation.

ADDITIONAL INFORMATION

Special stains and cultures may be indicated, especially for the diagnosis of infectious processes. Brushings are much more useful for the diagnosis of neoplasms than for diagnosing inflammatory or infectious processes. For gastrointestinal tract cytology, non-diagnostic if the specimen is contaminated with food or barium sulfate.

METHODOLOGY

Preparations are examined cytologically using the Pap stain method.