Cerebrospinal Fluid Cytology
NAME OF PROCEDURE:
Cerebrospinal Fluid Cytology
88108 – Cytopathology, concentration technique, smears and interpretation
CSF, Spinal Fluid Cytology
Hydrocele Fluid Cytology, Lumbar Tap Cytology, Ventricular Tap Cytology
- Information regarding type of specimen, admitting diagnosis, and pertinent clinical history (i.e., age, clinical impression, past diagnosis, radiographic findings, and history of radiation/chemotherapy or malignancy) is essential to interpretation and should be noted on the
- Infectious diseases suspected and special stains requested should be
- Clinical evidence of immunosuppression should be indicated.
- Special handling requirements (isolation) should be clearly noted on the requisition.
- If other studies are required, submit to appropriate laboratory separately.
- Fresh Cerebrospinal FluidVolume: 3 – 5 mL Minimum Volume: 1 mL
Cytologic examination of CSF is used to investigate patients with sign or symptoms of central nervous system disease.
- Sterile, clean, leak-proof container.
- Label container(s) with a minimum of two patient identifiers.
- Complete the requisition entirely.
- Pre-label collection container(s) with patient name and sample site.
- Using standard CSF procedure, collect a minimum of 1 mL of CSF in labeled, leak proof container.
- Seal container well to prevent leakage
- Placed labeled container into a Biohazard specimen bag and include the requisition in the outside pocket of the bag.
- Send the entire specimen to the laboratory, STAT.
- Do not add anticoagulant or fixative.
- REFRIGERATE fresh/unfixed specimen if immediate delivery to Lab/Cytology is not possible.
- Transport with ice pack being careful not to freeze specimen.
**REFRIGERATE ALL CYTOLOGY SPECIMENS IF IMMEDIATE DELIVERY TO LAB/CYTOLOGY IS NOT POSSIBLE**
Anatomical Pathology Requisition
- Complete patient information section.
- Complete the Non-Gynecologic section thoroughly.
CAUSES FOR REJECTION:
- Incomplete requisition.
- Specimen container not labeled properly.
- Gross contamination due to spillage.
- Prolonged period (more than 8 hours) at room temperature.
- Delay in delivery for adequate processing.
- Cells may rapidly degenerate rendering morphologic evaluation less than optimal if adequate care is not taken.
- Generally, morphology of cells can be adequately maintained with prompt refrigeration for 24 hours.
- Cytocentrifuge preparations are examined microscopically using the Pap stain method and Wright-Giemsa stain method.
- Pneumoencephalography, myelography, radiation, or intrathecal therapy can produce striking cytologic changes.
- Meningeal carcinomatosis or lymphoma may shed few cells, intermittently.
- Malignant cells are shed into CSF only from tumors which extend to subarachnoid space or the ventricles.
- Metastatic tumors have a better detection rate than primary tumors.
- Material for Flow Cytometry should be collected in RPMI and submitted separately.
Information is also available on the SEPA website under Resources – Directory of Services.
Please call laboratory if you have questions.